May 1, 2013 The core NHEJ machinery includes the end-binding heterodimeric proteins Ku70 /Ku80, the DNA-PKcs protein kinase, and the complex

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In the presence or absence of Ku70 overexpression, 293T cells grown in serum from CR animals were less susceptible to Bax-mediated apoptosis as compared to cells grown in media with AL serum ( Fig. 1D ). The Ku70 NLS contains five lysines (K539, K542, K544, K553 and K556) that were identified as targets for acetylation in vivo. 18, 29 Lysine acetylation in the cytoplasm drives and coordinates key events such as cytoskeleton dynamics, intracellular trafficking, vesicle fusion, metabolism and stress response. 43, 44 We hypothesized that acetylation of the five lysine residues in the Ku70 NLS 2001-03-01 · Ku70:Ku80 complex Source: GO_Central "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium." Gaudet P. , Livstone M.S. , Lewis S.E. , Thomas P.D. Brief Bioinform 12:449-462(2011) [ PubMed ] [ Europe PMC ] [ Abstract ] Anti-Ku70 Antibody (E-5) is a mouse monoclonal IgG 1 κ Ku70 antibody, cited in 60 publications, provided at 200 µg/ml; raised against amino acids 302-609 mapping at the C-terminus of Ku70 of the Ku protein of human origin We present evidence that inactivation of the Ku70 gene leads to a propensity for malignant transformation both in vitro and in vivo. In vitro, Ku70−/− mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku70−/− mice, known to be defective in B but not T lymphocyte maturation, developed thymic Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

We further deﬁned the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and DNA-PKCS interaction. 2. Results 2.1. The Enhanced Cyan Fluorescent Protein (ECFP)-Ku70/EYFP-Ku80 FRET Pair The Ku70/80 heterodimer binds to DNA ends as a starting point for NHEJ complex assembly and juxtaposition Ku70 (E-5): sc-17789 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80). In addition to NHEJ, Ku70/80 also plays an important role in multiple biological processes including telomere maintenance [], HIV replication [] and suppression of apoptosis [].

Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications.

The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria. However, the molecular mechanism underlying Ku70-dependent inhibition of Bax is not fully understood. Here, we show that the absence of Ku70 results in the accumulation of ubiquitylated Bax. Under normal growth conditions, Bax ubiquitylation

Rabbit anti Ku70 antibody recognizes human Ku70, also known as XRCC6, G22P1 or X-ray repair cross-complementing protein 6. Ku70 is involved in repair of double stranded DNA breaks via the non-homologous end joining (NHEJ) pathway. Ku70 forms a heterodimer with Ku80 (Ku70/Ku80), which binds to the ends of broken DNA where it recruits proteins for end-processing and ligation.

Eukaryotic Ku is a heterodimer of two polypeptides, Ku70 (XRCC6) and Ku80 (XRCC5), so named because the molecular weight of the human Ku proteins is around 70 kDa and 80 kDa. The two Ku subunits form a basket-shaped structure that threads onto the DNA end.

KU70.

Contents. 1 Function; 2 Aging; 3 Clinical; 4 Nomenclature; 5 Interactions; 6 References Jun 1, 2001 Background and Purpose—Ku70 and Ku86, multifunctional DNA repair proteins, bind to broken DNA ends, including double-strand breaks, Anti-Ku70 Protein Antibody from rabbit; Synonym: X-ray repair cross- complementing protein 6, 5′-deoxyribose-5-phosphate lyase Ku70, 5′-dRP lyase Ku70, Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK).
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Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system. Royaltyn eller avgiften ska då i stället redovisas på KU10. Att royaltyn ska redovisas som tjänst gäller däremot inte om den som fått royaltyn inte ska betala skatt i Sverige och inte heller omfattas av svensk socialförsäkring.

KU70 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications.
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Ku70 (also known as XRCC6) is a eukaryotic protein that is involved in the repair of DNA double-strand breaks by non-homologous end-joining [ 1, 2 ] Ku is a heterodimer of approximately 70kDa and 80kDa subunits

Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96]. It is a heterodimeric protein made up of Ku70 and Ku80 [97] .

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Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex.

1 Function; 2 Aging; 3 Clinical; 4 Nomenclature; 5 Interactions; 6 References Jun 1, 2001 Background and Purpose—Ku70 and Ku86, multifunctional DNA repair proteins, bind to broken DNA ends, including double-strand breaks, Anti-Ku70 Protein Antibody from rabbit; Synonym: X-ray repair cross- complementing protein 6, 5′-deoxyribose-5-phosphate lyase Ku70, 5′-dRP lyase Ku70, Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three Ku70 or Ku80 overexpression by transfection with the Ku70 or Ku80 expression gene, respectively, enhanced proliferation of cells with low NF-κB levels.

Jun 22, 2010 Ku70 and Ku80 are part of a high‐affinity heterodimeric protein complex, known as Ku (19, 27). The Ku heterodimer binds nonspecifically to DNA

These Rabbit Polyclonal Ku70 antibody. Validated in WB, ICC/IF, IHC-P, IP, PLA. Tested in Human, Mouse. Cited in 11 reference(s). Independently reviewed in 1 Unconjugated Whole IgG Rabbit anti-Ku70 Antibody, Affinity Purified suitable for WB, IP applications.

To understand the roles of CBP and Ku70 in human melanoma cells, we designed and synthesized a set of CBP siRNA and Ku70 Although the ku70 deletion strain was noted to be more sensitive to UV rays than the corresponding wild-type strain, no lethality, severe growth retardation or loss of gene copy numbers could be detected during repetitive rounds of cultivation and induction of heterologous protein production.